Abstract: OBJECTIVE To explore the distribution of pathogens isolated from ear canal secretions of 120 patients with chronic suppurative otitis media and observe the effect of antimicrobial treatment so as to provide guidance for treatment of chronic suppurative otitis media.METHODS A total of 120 patients with chronic suppurative otitis media who were treated in the hospital from Jan 2014 to Jun 2016 were enrolled in the study and randomly divided into the observation group and the control group, with 60 cases in each group.The control group was given the conventional therapy, while the observation group was treated with combined drug therapy.The constituent ratios of the pathogens cultured from the ear canal secretions and the drug resistance rates of the pathogens were observed and recorded; the hearing recovery status, adverse reactions, and clinical curative effect were compared between the two groups of patients.RESULTS The pathogens were cultured from the ear canal secretions of 118 in 120 ears, the bacteria were cultured from 116 ears, accounting for 98.30%, including 74 (62.71%) strains of gram-positive bacteria and 42 (35.59%) strains of gram-negative bacteria; the fungi were cultured from 2 ears, accounting for 1.70%.The analysis of the drug resistance of the pathogens showed that the gram-positive bacteria and the gram-negative bacteria varied in the drug resistance; among the gram-positive bacteria, the Staphylococcus spp was highly susceptible to cefazolin, erythromycin and netilmicin; among the gram-negative bacteria, the Pseudomonas aeruginosa was highly susceptible to ceftazidime, vancomycin, ceftriaxone, and ampicillin.Both the gram-positive bacteria and gram-negative bacteria were susceptible to gentamicin and ciprofloxacin.The improvement rate of hearing of the observation group was 95.00%, significantly higher than 81.67% of the control group(P＜0.05).The incidence of adverse reactions of the observation group was 10.00%, significantly lower than 23.33% of the control group (P＜0.05).The total effective rate of treatment of the observation group was 91.67%, significantly higher than 73.33% of the control group (P＜0.05).CONCLUSIONThe S.aureus, S.epidermidis, and P.aeruginosa are dominant among the pathogens isolated from the patients with chronic suppurative otitis media, there are a variety of antibiotics, and the pathogens vary in the drug resistance, therefore, it is necessary to reasonable use antibiotics based on the bacterial culture and result of drug susceptibility testing so as to avoid the drug abuse and treat the patients more accurately.