Abstract: OBJECTIVE To investigate the prevalence of Candida and Trichomonas vaginalis infection in vaginal secretions of patients with senile vaginitis so as to provide more evidence-based medical evidence for prevention and treatment of the Candida and T.vaginalis infection in vaginal secretions.METHODS A total of 546 patients with senile vaginitis who were treated in the hospital from Jun 2013 to Jun 2016 were enrolled in the study, and the provenance rate of Candida and T.vaginalis infection was detected by using microscope.RESULTS Of the 546 patients with senile vaginitis, 114 (20.9%) had Candida infection, and 132 (24.2%) had T.vaginalis infection.The prevalence rate of Candida infection was 31.4% in the patients aged no more than 70 years old, 12.7% in the patients aged between 71 and 80 years old, 5.4% in the patients aged no less than 81 years old, and there was significant between-group difference (P＜0.05).The prevalence rate of T.vaginalis infection was 37.5% in the patients aged no more than 70 years old, 14.4% in the patients aged between 71 and 80 years old, 1.8% in the patients aged no less than 81 years old, and there was significant between-group difference (P＜0.05).Among the patients with senile vaginitis, the incidence rate of Candida infection was 32.8% in peasants, 16.9% in self-employed businessmen, 9.4% in enterprise employees, and there was significant between-group difference (P＜0.05); the incidence rate of Candida infection was significantly higher in peasants than in self-employed businessmen (P＜0.05), and the incidence rate of Candida infection was significantly higher in self-employed businessmen than in enterprise employees (P＜0.05).Among the patients with senile vaginitis, the incidence rate of T.vaginalis infection was 19.4% in peasants, 31.9% in self-employed businessmen, 16.0% in enterprise employees; the incidence rate of T.vaginalis infection was significantly lower in peasants and enterprise employees than in self-employed businessmen (P＜0.05).The incidence rate of Candida infection was 36.0% in spring, 17.4% in summer, 13.9% in autumn, 12.5% in winter; the incidence rate of Candida infection was significantly higher in spring than in summer, autumn, and winter (P＜0.05).CONCLUSIONThe prevalence rates of Candida and T.vaginalis are high in vaginal secretions of the patients with senile vaginitis.It is necessary to take targeted prevention measures so as to prevent and treat the Candida and T.vaginalis infection.