Abstract: OBJECTIVE To explore the prevalence of human papillomavirus (HPV) infection in women with different heath state and observe the distribution of its subtypes so as to provide guidance for diagnosis and treatment of HPV infection as well as prevention of cervical cancer.METHODS A total of 22 419 women who received physical examination in physical examination center, were treated in gynecology outpatient department, and were diagnosed with cervical cancer in oncology department of Affiliated Hospital of Zunyi Medical College from Jan 2015 to Dec 2015 were enrolled in the study, and the genotypes of HPV were detected by using rapid flow-through hybridization typing of nucleic acid molecules.RESULTS The incidence rate of HPV infection was the highest (84.42%) in the patients confirmed with cervical cancer, of whom 83.50% had single infection; the incidence rate of HPV infection was 37.47% in the women who were treated in the gynecology outpatient department, of whom 80.36% had single infection; the incidence rate of HPV infection was 13.24% in the women who received the physical examination, of whom 86.30% had single infection, and there was significant difference in the incidence rate of HPV infection among the three groups (P＜0.01).The HPV subtypes varied among the three groups, the high-risk HPV subtype infection was the most prevalent; the HPV52, 58, and 16 ranked the top 3 subtypes of HPV among the women who received the physical examination, accounting for 19.36%, 11.28%, and 11.02%, respectively.The HPV52, 16, and 58 ranked the top 3 subtypes of HPV among the women who were treated in the gynecology outpatient department, accounting for 17.92%, 14.54%, and 10.97%, respectively.The HPV16, 52, and 18 ranked the top 3 subtypes of HPV among the patients who were confirmed with cervical cancer, accounting for 33.88%, 28.10%, and 19.00%, respectively.The incidence rate of HPV infection was relatively high among the physical examination women who were aged more than 51 years old (P＜0.01); among the women who were treated in the gynecology outpatient department, the incidence rate of HPV infection was 69.39% in the women aged more than 61 years old, 53.61% in the women aged between 15 and 20 years old (P＜0.01); however, there was no significant difference in the incidence rate of HPV infection among the patients confirmed with cervical cancer in different age groups.CONCLUSIONThe HPV infection is prevalent among the women under different health state, the single high-risk HPV infection is dominant and associated with age.It is necessary to strengthen the screening of HPV among the different age groups of women so as to prevent the cervical cancer.