Abstract: OBJECTIVE To investigate the prevalence rate of nosocomial infections, use of antibiotics, isolation rates of pathogens and related factors for the infection in Daqing so as to control the nosocomial infections and reduce the incidence of nosocomial infection.METHODS By means of cross-sectional survey, the related,unified trainings were conducted for full-time infection control staff, the prevalence rate of nosocomial infections in the patients who were hospitalized from 0:00 to 24:00 on May 18, 2016 was investigated, the questionnaires were filled out, and the statistical analysis was performed.RESULTS Totally 17 hospitals of Daqing were investigated, 7327 hospitalized patients were supposed to be investigated, 7274 of whom were actually investigated, with the actual investigation rate 99.28%. Totally 140 patients had nosocomial infections, with the prevalence rate 1.92%; totally 1404 patients had community-acquired infections, with the prevalence rate 19.30%. The patients with lower respiratory tract infections were dominant among the patients with nosocomial infections. Among the patients with infection in tertiary hospitals, the patients with lower respiratory tract infections accounted for 52.05%, the patients with upper respiratory tract infections 19.18%, the patients with urinary tract infections 12.33%. The submission rate of specimens was 61.22%, the utilization rate of antibiotics was 25.17%; the patients who were treated with antibiotics for therapy accounted for 76.08%, the patients who were treated with prophylactic antibiotics accounted for 7.10%, and the patients who were treated with therapeutic and prophylactic antibiotics accounted for 16.82%. CONCLUSION The survey of prevalence rate of nosocomial infections actually reflect the prevalence and development trends in Daqing. It is necessary to strengthen the surveillance of nosocomial infections in key departments and boost the management of antibiotics so as to effectively reduce the incidence of nosocomial infections.