Abstract: OBJECTIVE To investigate dynamic monitoring features of multiple biochemical indexes in patients with chronic hepatitis C infection. METHODS A total of 80 cases of patients with chronic hepatitis C infection in our hospital from Dec. 2012 to Dec. 2014 were randomly selected as the study objects. Based on the number of serum viral load of chronic hepatitis C infection, they were divided into high replication group (36 cases) and low replication group (44 cases). A number of biochemical indexes including ALT, AST and CHE of patients were detected and analyzed, and the HCV-RNA and anti -HCV positive detection rates at the different stages of the patients were analyzed. RESULTS There was no significant difference in ALT, AST and CHE between the two groups before treatment. After treatment, the ALT and AST of high replication group were (75.21 ± 12.38) U/L and (52.35 ± 12.84) U/L, which were higher than (61.22 ± 18.57) U/L and (31.32 ± 8.64) U/L of low replication group, and the differences were significant (P<0.05). The CHE of high replication group was (2867.48 ± 123.56) U/L, which was significantly lower than (3564.12 ± 211.45) U/L of low replication group (P<0.05). The positive rates of HCV-RNA of the 80 cases of patients with chronic hepatitis C at different stages before and after treatment were significantly different (P<0.05). CONCLUSION In patients with chronic hepatitis C infection, once the infection occurs, the anti-HCV indexes will be positive for a long time, and HCV-RNA level which change with the development of the patient's condition, the higher the more severe of its function in patients with liver damage.