Abstract: OBJECTIVE To analyze the diagnostic value of detection of albumin, prealbumin and C- reactive protein (CRP) in surgical site infections in department of general surgery patients after operation. METHODS A total of 320 cases of general surgery patients with operation from Feb. 2014 to Feb. 2016 were selected. All the patients were diagnosed without infections. Blood samples were taken at postoperative 12h, 24h, 3d and 7d for detection of albumin, prealbumin and CRP, respectively. All patients were monitored for infections within 7d after operation. According to the results of the diagnosis, the patients were divided into infection group (27 cases) and non-infection group(293 cases), and the levels of albumin, prealbumin and CRP at different time points before and after operation between the two groups were compared. RESULTS There were 27 cases of postoperative infections, and the infection rate was 8.44%. Totally 36 strains of pathogens were detected, of which 10 strains were gram-negative, accounting for 27.78%, 16 strains were gram-positive, accounting for 44.44%, and 10 strains were fungi, accounting for 27.78%. The levels of albumin, prealbumin and CRP in preoperative 2 h between the two groups showed no significant difference. The levels of albumin, prealbumin and CRP at postoperative 12 h, 24 h, 3d and 7d of the two group were significantly lower than those before operation, and those in infection group were significantly lower than in non-infection group (P＜0.05). The levels of CRP at postoperative 12 h, 24 h, 3 d and 7d of the two group were significantly higher than those at preoperative 2h, and that in infection group was significantly higher than in non-infection group (P＜0.05). Comparison of the sensitivity and specificity of 7 diagnostic schemes of albumin, prealbumin, CRP, albumin+prealbumin, albumin+CRP, prealbumin+CRP and albumin+prealbumin+CRP in the diagnosis of postoperative infection, albumin+prealbumin+CRP had the best sensitivity and specificity. CONCLUSION Using albumin+prealbumin+CRP experimental data for diagnosis of surgical site infection in department of general surgery patients, timeliness and objectivity are good, which has certain clinical value.