Abstract: OBJECTIVE To find the best drug solution concentration and the most effective lock time in the regular and common lock time and drug concentration of antibiotic lock technology (ALT). METHODS Silica gel film samples containing bacterial biofilms were randomly divided into four different concentration groups, which were 300×MIC, 600×MIC, 900×MIC and blank control group, with 5 samples in each group, silica gel films were taken out after intervention at 6 h, 12 h, 18 h and 24 h, quantitative culture of bacteria was carried out by dilution plate count method, and the most effective lock time and concentrations of ALT were compared. RESULTS The most effective time under different concentrations: silicone film bacteria count under 300×MIC and the control group had no significant difference, bacteria count results appeared significant decline at 6h under 600×MIC(P<0.05), while no significant difference at 12 h and at 6 h, at 18 h and at 24 h , and bacteria count had significant difference at 18h and at 6 h, at 12 h (P<0.05); bacteria count results appeared significant decline at 6h under 900×MIC(P<0.05), bacteria count had significant difference at 6h and at 12 h (P<0.05), and had no significant difference at 12 h and at 18 h, at 24 h . The most effective concentration under different time lock: at the same locked time, bacteria count under 300×MIC and the control group had no significant difference, bacteria count was dramatic decline under 600×MIC and 900×MIC, which had significant difference(P<0.05), and bacteria count under 600×MIC and 900×MIC had significant difference (P<0.05). CONCLUSION The 300×MIC as ALT solution has no ability to remove bacteria biofilm, but 600×MIC and 900 ×MIC as ALT solution have good ability to remove bacteria biofilm. In a certain lock time, the higher the concentration, the stronger its ability to remove biofilm. The lock time of 18 h and 12 h for 600×MIC and 900 ×MIC can achieve the best removal effect, respectively.