Abstract: OBJECTIVE To investigate and analyze the correlation of bacterial resistance and the antibiotic usage. METHODS The bacterial resistance and defined daily doses (DDDs) in our hospital from Jan. 2010 to Dec. 2016 were analyzed, and the correlation between them was analyzed by Pearson method. RESULTS From 2010 to 2016, the top 3 antibacterial agents DDDs in our hospital were cehpalosporins, macrolides and quinolones. The common gram-negative bacteria was Escherichia coli and Klebsiella pneumoniae, and the resistant rate of E.coli showed a positive correlation with the DDDs of cefepime and amikacin (P＜0.05). The common gram-positive bacteria was Staphylococcus aureus and coagulase-negative Staphylococcus, and the resistant rate of S. aureus showed a positive correlation with the DDDs of clindamycin(P＜0.05). CONCLUSION The bacterial resistant rate is correlated with the antibiotic DDDs, and the rational use of antibiotics can reduce the bacterial resistant rate.