Abstract: OBJECTIVE To investigate the relationship between serum IFN-γ, IL-10 and gamma immunoglobulin with opportunistic infections in patients with human immunodeficiency virus (HIV)/(AIDS). METHODS A total of 50 cases of HIV/AIDS patients from Jun. 2015 to Jun. 2016 were selected as the research objects, and enzyme-linked immunosorbent assay (ELISA) was used to determine the serum levels of IFN-γ, IL-10 and gamma immunoglobulin. RESULTS Among 50 cases of HIV/AIDS patients, 40 cases had opportunistic infection, accounting for 80%, including 11 cases of single opportunistic infections (27.50%), and 29 cases of 2 or more than 2 kinds of opportunistic infections (72.50%). The levels of IL-10 and IgG in the patients with opportunistic infections were (95.55±158.41) and (28.83±3.24)pg/ml, which were higher than (10.22±19.10) and (11.24±2.22)pg/ml in uninfected group (P<0.05), and the IFN-γ level in the patients with opportunistic infections was (5.81±1.32) pg/ml, which was lower than (8.62±1.01)pg/ml in uninfected patients, and the difference was significant(P<0.05). IL-10 in the patients with Pneumocystis infection was (19.71±27.82)pg/ml, which was lower than (71.89±161.02)pg/ml in uninfected group (P<0.05). IgG levels in patients with Pneumocystis infection, Mycobacterium tuberculosis, Penicillium marneffei and other fungi infection were higher than that in uninfected group (P<0.05). IgA and IgM levels in patients of opportunistic infection group and uninfected group had no significant difference. Among 50 cases of patients, 39 cases were improved, 7 cases were deterioration or no change, and 4 cases died. CONCLUSION Serum IL-10 and IFN-γ may be closely related to the occurrence of opportunistic infections in HIV/AIDS patients, low levels of IL-10 can reduce the body's ability to remove Pneumocystis, and IL-10 and IFN-γ have no obvious relation with disease prognosis.