Abstract: OBJECTIVE To explore the expression of neural Wiskott-Aldrich syndrome protein (N-WASP) in clear cell renal cell carcinoma and normal renal tissue adjacent to carcinoma and its correlation with tumor pathological grade and clinical stage. METHODS A total of 70 patients with clear cell renal cell carcinoma from Jun. 2012 to Jun. 2014 in our hospital were collected and analyzed. All the patients underwent radical nephrectomy or partial nephrectomy in our hospital. The specimens were made into renal clear cell carcinoma tissue microarray and adjacent normal renal tissue microarray, with 70 cases in each group. All patients were followed up for 3 years. The immunohistochemical staining was used to compare the difference of N-WASP expression between two kinds of chips. The correlation between the expression level of N-WASP and tumor pathological grade and clinical stage was analyzed. Univariate analysis and multivariable analysis of survival rate in patients with clear cell renal cell carcinoma were carried out. RESULTS Cancer tissue N-WASP expression level was (75.54±8.48), which was significantly lower than (121.85±10.46) (P<0.05). After 3 years of follow-up, the survival rates of weak and moderate level of N-WASP was 80.95%, which was higher than 25.00% strong level of N-WASP (P<0.05). Age and tumor diameter were the related factors of survival in patients with clear cell renal cell carcinoma (P<0.05). N-WASP expression level was an independent factor of survival in patients with clear cell renal cell carcinoma (P<0.05). CONCLUSION The expression level of N-WASP in renal clear cell carcinoma was decreased, which was an independent predictor of survival in patients with clear cell renal cell carcinoma.