Abstract: OBJECTIVE To investigate the expression of E-cadherin in trophoblast cells of pregnant women with recurrent miscarriage during early pregnancy and to analyze the correlation between E-cadherin and human papillomavirus (HPV) infection. METHODS The clinical diagnosis and treatment data of 120 pregnant women with recurrent miscarriage during early pregnancy from Jan. 2016 to Dec. were selected as observation group, another 120 pregnant women with normal early pregnancy were set as control group. The villus samples of the two groups were collected, and the expression of E-cadherin in villous trophoblast cells was detected. The HPV-DNA infection was also detected in the two groups. RESULTS The expression of E-cadherin was positive in both groups. There were 69 cases of E-cadherin positive in observation group with the positive rate of 57.50%, which was significantly lower than 111 cases in control group, with the positive rate of 92.50% (P<0.05). There were 42 cases of HPV infection in observation group with the infection rate of 35.00%, which was significantly higher than 15 cases in control group, with the infection rate of 12.50% (P<0.05). The patients with HPV16 infection accounted for 85.71% among the patients with HPV infection in the observation group, which was significantly higher than 40.00% in control group (P<0.05). There were 15 cases of E-cadherin positive in pregnant women with HPV infection, with the E-cadherin positive rate of 35.71%, which was significantly lower than 15 cases in control group, with E-cadherin positive rate of 100.00% (P<0.05). The correlation between E-cadherin expression and HPV infection was negatively correlated (P＜0.05). CONCLUSION E-cadherin has positive expression in trophoblast cells of pregnant women during early pregnancy, and E-cadherin positive expression may be involved in the process of recurrent miscarriage. Pregnant women infected with HPV, especially HPV16 may cause abnormal expression of E-cadherin.