Abstract: OBJECTIVE To explore the effects of genital herpes virus infection on immune function and sexual life in patients. METHODS A total of 102 male patients with genital herpes virus infection from Jan. 2014 to Feb. 2016 treated in our hospital were selected as observation group, and 102 healthy men undergoing physical examination at the same period were set as control group. The subcellular markers such as CD₃⁺T, CD₄⁺T, CD₈⁺T, CD₄⁺T/CD₈⁺T, B cell(B), and natural killer cell (NK) were detected, and the differences between the two groups were analyzed. The international erectile dysfunction scale and the international ejaculatory dysfunction evaluation scale were used to evaluate the sexual function of all the subjects. RESULTS The number of premarital sexual contact in observation group was 68 cases (66.67%), which was more than 35 cases (34.31%) in control group (P＜0.05). The CD₃+, CD₄⁺ and CD₈⁺T in observation group were (60.23±11.28)%, (35.67±9.68)% and (22.39±9.23)%, which were significantly lower than (74.67±13.07)%, (42.11±10.32)% and (29.24±10.38)% in control group. The CD₄⁺T/CD₈⁺T in observation group was (1.59±0.37), which was significantly higher than (1.44±0.37) in control group (P＜0.05). The grade Ⅰ, Ⅱ and Ⅲ erectile dysfunction and ejaculatory dysfunction between the two groups had significant differences (all P＜0.05). CONCLUSION Male genital herpes virus seriously affects the immune level and sexual life, and immune level and sexual life level are lower than those of normal healthy men, so we should develop appropriate programs for treatment.