Abstract: OBJECTIVE To investigate the effect of centralized management of reusable tracheal cannula in sterilization and supply center, so as to reduce the infections caused by unqualified cleaning and disinfection of tracheal cannula. METHODS A total of 40 tracheal cannulas with decentralized treatment by the clinical departments and 100 tracheal cannulas treated in sterilization and supply center with pretreatment, manual cleaning, ultrasonic cleaning machine cleaning, full automatic cleaning machine cleaning, high pressure air gun drying, drying cabinet drying, paper and plastic bags packing, and high-pressure steam sterilization pot sterilization from Jan. 2015 to Dec. 2015 were selected and sampled for visual and bacteriological tests, and the qualified rates at different periods during the storage of 180 days were compared. RESULTS After the decentralized treatment of clinical departments, using random sampling for visual and bacteriological tests, the qualified rates were 95.00% on the first days, 87.50% on the fourth day, 80.00% on the seventh day, and 50.00% on the tenth day. After the centralized treatment in disinfection and supply center, the qualified rates of paper and plastic bags packing were all 100% within 180 days after the independent packing. The differences were significant (P<0.05). CONCLUSION The centralized management of reusable tracheal cannula in sterilization and supply center can ensure the sterilization quality and patient safety, and help to control nosocomial infections, at the same time independent packaging can extend the preservation time, and reduce manpower and material resources.