Abstract: OBJECTIVE To investigate the prevalence and distribution of nosocomial infections in a hospital from 2014 to 2016 so as to provide guidance for prevention and control of nosocomial infections.METHODS By means of the hospital information system (HIS) and laboratory information system (LIS), the prevalence of nosocomial infections in the patients who were hospitalized from 2014 to 2016 was retrospectively and statistically analyzed.RESULTS Of totally 94953 hospitalized patients who were monitored from 2014 to 2016, 1058 had nosocomial infections, with the infection rate 1.11%. Totally 891 patients were submitted, with the submission rate 84.22%. The nosocomial infection was highly prevalent in the department cardiac surgery (including postoperative care unit); the lower respiratory tract was the predominant infection site, followed by the bloodstream, upper respiratory tract, urinary tract and surgical incision sites. A total of 567 strains of pathogens were isolated from the patients with nosocomial infections from 2014 to 2016, 73.72% of which were gram-negative bacteria, 15.70% were gram-positive bacteria, and 10.58% were fungi; Klebsiella pneumoniae (145 strains, 25.57%), Acinetobacter baumannii (71 strains, 12.52%), Pseudomonas aeruginosa (47 strains, 8.29%), Enterobacter cloacae (42 strains, 7.41%) and Candida albican (37 strains, 6.53%) ranked the top 5 species of pathogenic bacteria. Of the specimens sources, 62.96% were sputum specimens, 19.30% were blood specimens, and 6.85% were urine specimens.CONCLUSION The nosocomial infection is highly prevalent in the department of cardiac surgery, the lower respiratory tract, blood and upper respiratory tract are the predominant infection sites, the gram-negative bacteria are dominant among the isolated pathogens, and the K.pneumoniae is the predominant species of pathogen.