Abstract: OBJECTIVE To investigate the predictive effect of serum β-human chorionic gonadotropin (β-hCG) and C-reactive protein (CRP) on intrauterine infection in pregnant women with premature rupture of membranes. METHODS From Oct. 2014 to Dec. 2016, 108 patients with premature rupture of membranes were enrolled in this study. According to whether the combined intrauterine infection, patients were divided into infection group (n=46) and non-infection group (n=62). The prenatal levels of serum β-hCG and CRP were measured, and placentas were collected for postnatal examination. RESULTS The levels of serum β-hCG and CRP in infection group were (5.56±1.63)ng/ml and (26.25±12.58)mg/L, which were significantly higher than those in non-infection group (P<0.05). Serum β-hCG and CRP levels were gradually increased with the increase of infection degree, and the levels of serum β-hCG and CRP in patients with different intrauterine infection were significant (P<0.05). When the serum β-hCG ≥ 2.26 ng / ml and CRP ≥ 12.85 mg / L, the AUC on the diagnosis of intrauterine infection was the maximum.The sensitivity, specificity, positive predictive value and negative predictive value of β-hCG combined with CRP for the diagnosis of intrauterine infection were significantly higher than those of the single index (P<0.05). CONCLUSION Combined detection of serum β-hCG and CRP is of great value in the early diagnosis of intrauterine infection in pregnant women with premature rupture of membranes and is closely related to the prognosis of patients.