Abstract: OBJECTIVE To analyze the distribution and risk factors of multidrug-resistant bacteria in emergency intensive care unit (EICU). METHODS The clinical data of 88 hospitalized patients in EICU from Jan. 2016 to Dec. 2016 were retrospectively analyzed. The differences between multidrug-resistant group (42 cases) and non multidrug-resistant group (46 cases) were compared, the distribution characteristics of multidrug-resistant bacteria were analyzed, and related factors of multidrug-resistant bacteria infection were analyzed. RESULTS There were 42 strains of multidrug-resistant pathogenic bacteria, including 32 strains of carbopenems-resistant Acinetobacter baumanii (CR-AB) accounting for 76.19%, 5 strains of methicillin-resistant Staphylococcus aureus (MRSA) accounting for 11.91%, and 3 strains of carbopenems-resistant Enterobacteriaceae (CRE) accounting for 7.14%. The drug resistant rates of CR-AB to cefoperazone/shubatan and tigecycline were 18.75% and 0%, that of MRSA to tigecycline was 0.00%, and those of MDR-PA to cefoperazone/shubatan, piperacillin/tazobactam, imipenem, and tigecycline were all 0.00%. Hospitalization time >14d, antibiotics usage time >3d, central venous catheter, endotracheal intubation, indwelling catheter or tube, and mechanical ventilation time≥2d were related independent risk factors for EICU patients with multidrug-resistant bacteria infection (P<0.05). CONCLUSION Multidrug-resistant bacteria infection in NICU patients is the result of multiple factors, clinical comprehensive and effective measures should be taken to reduce the multidrug-resistant bacteria infection in NICU patients, and to prevent the spread of multidrug-resistant bacteria.