Abstract: OBJECTIVE To investigate the distribution and drug resistance of Klebsiella pneumoniae(KPN) in patients in intensive care unit(ICU), so as to provide evidence for clinical infection control. METHODS A total of 551 strains of KPN isolated from ICU from Jan. 2014 to Dec. 2016 were selected, and pathogenic bacteria identification and drug susceptibility test were carried out. RESULTS The detection rates of KPN in ICU were 16.29%, 14.56% and 15.25% in 2014-2016, respectively. The total detection rate was 15.35% in three years. The main specimens were from sputum and blood. The resistant rates of KPN to ampicillin, ceftriaxone, ampicillin sulbactam, aztreonam and ceftazidime were 99.82%, 57.71%, 56.81%, 49.36% and 42.65%, with a decline trend, and the resistant rates to imipenem were 39.88%, 33.55% and 17.32% in the three years, showing a significant decline. CONCLUSION The detection rate of KPN infection in ICU is not increased for the 3 consecutive years, and the drug resistant rate is decreased year by year. It is suggested to strengthen the monitoring of multidrug-resistant bacteria and the use management of antimicrobial agents, and choose sensitive antimicrobial agents according to drug susceptibility results to reduce the proliferation of KPN.