Abstract: OBJECTIVE To investigate the prevalence of extraintestinal infection with Aeromonas hydrophila, related factors and drug resistance so as to provide guidance for clinical prevention and treatment of the A.hydrophila infection.METHODS Totally 112 strains of A.hydrophila were isolated from extraintestinal specimens of 112 patients who were treated in the hospital from Jan 2009 to Dec 2016. The specimens sources, seasonal distribution and drug resistance were observed.RESULTS The extraintestinal A.hydrophila infection was highly prevalent in autumn and led to infection of sterile sites. The patients with wound infection accounted for 40.18%, the patients with blood infection 31.25%. The history of surgery and drainage tube indwelling were the major clinical characteristics of the patients. The extraintestinal A.hydrophila strains were highly susceptible to fourth generation cephalosporins, carbapenems, aminoglycosides and quinolones, however, the multidrug-resistant strains have emerged, the isolation rate of carbapenem-resistant strains showed an upward trend.CONCLUSION The extraintestinal A.hydrophila infection is highly prevalent among elderly men and is most common in autumn, and it is necessary for the hospital to pay great attention to the drug resistance and the carbapenem-resistant strains.