Abstract: OBJECTIVE To explore the operating room-related factors for surgical incision infections and observe the effect of intervention measures so as to provide guidance for effective prevention of postoperative infections. METHODS A total of 2210 patients who received surgical procedures in hospitals from Jan 2014 to Dec 2016 were recruited as the study objects, 1026 patients who were not treated with interventions from Jan 2014 to Jun 2015 were assigned as the control group, while 1184 patients who were treated with interventions from Jul 2015 to Dec 2016 were set as the study group.The prevalence of postoperative incision infections was investigated, the univariate analysis and multivariate logistic regression analysis were performed for the operating room-related influencing factors, and the incidence of incision infections, average length of hospital stay and hospitalization cost were observed and compared between the two groups of patients before and after the intervention. RESULTS The operation duration, surgical incision, non-laminar flow surgery, number of surgical participants and bacterial colony counts in air were the major operating room-related factors for the postoperative incision infections.The incidence rate of infection was 7.60% (78/1026) in the control group, 2.20% (26/1184) in the study group after the intervention, and there was significant difference between the two groups (P＜0.05).The length of hospital stay of the study group was (7.87±3.060) weeks, significantly shorter than (12.94±5.63) weeks of the control group; the hospitalization cost of the study group was (10.62±2.39) ten thousand yuan, less than (15.32±3.57) ten thousand yuan of the control group (all P＜0.05). CONCLUSION The incidence of incision infections is high in the patients after surgical procedures, the operating room-related influencing factors include the operation duration, surgical incision, non-laminar flow surgery, number of surgical participants and bacterial colony counts in air.It is necessary to take targeted prevention and treatment countermeasures according to the influencing factors so as to effectively reduce the incidence of incision infections, shorten the length of hospital stay and reduce the hospitalization cost.