Abstract: OBJECTIVE To discuss the clinical significance and related factors of objective monitoring in catheter-related hospital infections in ICU. METHODS A total of 600 patients with catheter therapy in ICU from Jan. 2015 to Dec. 2016 were included in the study, the objective monitoring of catheter-related hospital infections was performed from January 2016. The 300 patients treated in 2015 were set as control group, and 300 patients treated in 2016 were set as experimental group. The catheter-related hospital infection rate was compared between the two groups, and the pathogens of the catheter-related hospital infection were analyzed. RESULTS The patients in experimental group had 26 cases of catheter-related hospital infections with the infection rate of 8.67%, which were significantly lower than 42 cases and 14.0.% in 2015(P<0.05). The two groups were infected mainly by pulmonary and urinary tract infection. The infections of experimental group were 56 cases, which were less than 97 cases of control group (P<0.05). There were no significantly differences in the incidence of VAP, CAUTI, and CLABSI between the two groups. Totally 256 strains of pathogenic bacteria were detected in 70 cases of catheter-related hospital infections, including 146 strains of gram-negative bacteria accounting for 57.03%, mainly Acinetobacter baumannii and Klebsiella pneumonia, 86 strains of gram-positive bacteria accounting for 33.59%, mainly Staphylococcus epidermidis and Staphylococcus aureus, and 24 strains of fungi. CONCLUSION Objective monitoring can regulate the intubation operation and daily use of various catheters, thus effectively reduce the incidence of catheter-related hospital infections.