Abstract: OBJECTIVE To understand the distribution and drug resistance of Enterococcus spp strains in Hebei province so as to provide reliable basis for control of nosocomial infections. METHODS The Enterococcus spp strains that were isolated from 51 hospitals under Hebei antimicrobial resistance surveillance net were collected form Jan 1, 2015 to Dec 31, 2015, the strains were identified by using instrument method, the drug susceptibility testing was carried out with the use of Kirby-Bauer disk method or instrument method, and the statistical analysis was performed by means of WHONET5.6 software. RESULTS A total of 4159 strains of Enterococcus spp were isolated from the 51 hospitals in Hebei province, of which 2258 (54.3%) were Enterococcus faecium, 1575 (37.9%) were Enterococcus faecalis, 95 (2.3%) were Enterococcus avium, 86(2.1%) were Enterococcus gallinarum, 64 (1.5%) were Enterococcus casseliflavus, 51 (1.2%) were Enterococcus columbae, and 30 (0.7%) were other species.The drug resistance rate of the E.faecalis strains to vancomycin was the lowest (0.9%), and the drug resistance rate to erythromycin was the highest (74.5%); the drug resistance rate of the E.faecium strains to vancomycin was 2.4%, and the drug resistance rate to erythromycin was 87.7%; in addition, there was significant difference in the drug resistance rate between the Enterococcus strains isolated from urine specimens and the strains isolated from non-urine specimens. CONCLUSION The E.faecium and E.faecalis are the predominant species of Enterococcus causing the clinical infections and are highly resistant to antibiotics.It is necessary for the hospital to reasonably use antibiotics based on the result of the drug susceptibility testing.