Abstract: OBJECTIVE To explore the relationship between different genotypes of human papillomavirues (HPV) infections and cervical diseases so as to provide objective basis for prevention and treatment of cervical cancer. METHODS A total of 621 patients with cervical diseases who were treated in hospitals from Jan 2016 to Jan 2017 were enrolled in the study, the cervical epithelial cells were collected for liquid-based cytology test, and the HPV genotypes were identified by using nucleic acid molecular rapid hybridization genotyping technology. RESULTS Of the 621 patients with cervical diseases, 291 were positive for HPV, with the positive rate 46.86%.The number of positive cases and the positive rate were increased with the aggravation of degree of lesions.Among the 291 patients positive for HPV infections, the HPV16, HPV52, HPV58, HPV68 and HPV18 were the predominant HPV genotypes.With the aggravation of degree of lesions, the detection rates of low-risk HPV genotypes were decreased, while the detection rate of high-risk HPV genotypes were increased.The HPV16 genotype was closely associated with the degree of lesions, and the detection rate of the HPV16 genotype was significantly increased with the aggravation of degree of lesions (P＜0.01).There was no significant difference in the proportion of the patients with single infection or multiple infections among the different groups. CONCLUSION The HPV16, HPV52, HPV58, HPV68 and HPV18 are the most common HPV genotypes among the patients with cervical diseases, the detection rate of the HPV16 is positively correlated with the degree of lesions, and the multiple infections do not affect the degree of lesions.