Abstract: OBJECTIVE To investigate the diagnostic value of high sensitivity C-reactive protein (hs-CRP), procalcitonin (PCT) and serum amylase-like protein A (SAA) detection in neonatal early infection. METHODS A total of 90 cases of neonates admitted in our hospital from Jan. 2015 to Dec. 2016 were selected, and divided into two groups according to clinical manifestations and laboratory tests: bacterial infection group (54 cases) and virus infection group (36 cases). Fifty healthy neonates admitted to our hospital were selected as control group. Before treatment and during recovery (the 7th day after admission), bacterial infection group were taken venous blood, the control group were taken blood once at the time of admission to hospital. The levels of serum PCT, hs-CRP and SAA in the three groups were measured. And the sensitivity, specificity and accuracy of PCT, hs-CRP and SAA detection alone and together of early neonatal infection were analyzed. RESULTS The levels of hs-CRP, PCT, SAA and WBC were significantly increased in children with bacterial infection before treatment than those in other two groups (P<0.001), while the levels of PCT, SAA and WBC in children with viral infection were significantly higher than those in control group (P<0.001). After treatment, the levels of serum hs-CRP, PCT, SAA and WBC in bacterial infection group were (2.08±0.18)mg/L, (0.20±0.06)ng/ml, (0.96±0.13)ng/L and (10.59±2.47)×10⁹/L, which were significantly decreased (P<0.001). The levels of serum PCT, SAA and WBC in viral infection group after treatment were(0.15±0.28)ng/ml, (0.91±0.13)ng/L and (9.78±3.25)×10⁹/L, which were significantly decreased (P<0.05). The hs-CRP level in children with viral infection was significantly lower than that in children with bacterial infection (P<0.05). The serum levels of PCT, SAA and WBC in children with bacterial infection and viral infection after treatment showed no significant difference. The sensitivity, specificity and accuracy of combined detection of serum hs-CRP, PCT and SAA of neonatal infection were 95.00%, 97.50% and 100.00%, which were better than alone. CONCLUSION The combined detection of serum hypersensitive C-reactive protein, procalcitonin and serum amylase-like protein A significantly improves the sensitivity, specificity and accuracy of early diagnosis of neonatal infection, which had a certain clinical value.