Abstract: OBJECTIVE To investigate the infection situations and changes of serum tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6) and C-reactive protein (CRP) in patients with orthopedic infections. METHODS A total of 243 cases of orthopedic patients admitted to hospital from Jan. 2013 to Dec. 2016 were selected as the research objects. The infection situations, risk factors of infections and pathogens and the clinical inflammatory mediators of infected patients were taken for observation. RESULTS Totally 32 cases were with bone infections in the 243 cases of orthopedic patients, and the incidence of bone infection was 13.17%. Totally 54 strains of pathogens were detected, of which 35 strains were gram-negative bacteria accounting for 64.81% and 15 strains were gram-positive bacteria accounting for 27.78%. The duration of treatment, surgical treatment and hospitalization were the independent risk factors for bone infections in orthopedic patients (P<0.001). The levels of TNF-α, CRP, IL-12, IL-18, IL-6 and IL-8 in patients with bone infection were (5.99±1.31)mg/L, (24.11±7.61), (134.23±43.13), (253.11±50.12), (99.24±38.41) and (96.93±40.32)pg/ml, which were significantly higher than those in non-infected patients (P＜0.001). CONCLUSION The levels of serum tumor necrosis factor-α, C-reactive protein and related clinical factors in patients with bone infection are significantly increased. In the practice, patient-specific interventions should be given to the above-mentioned clinical features.