Abstract: OBJECTIVE To explore the value of CT combined with bronchoalveolar lavage fluid in the diagnosis of pulmonary fungal infection. METHODS A total of 128 patients with suspected pulmonary fungal infection from Mar. 2012 to Jun. 2016 were enrolled in this study and set as fungal infection group. Forty patients with common bacterial infection and forty patients of non-pulmonary infection were selected as bacterial infection group and non-infection group. The imaging features of patients with CT were observed and recorded in detail. The types of fungal infections, infectious diseases and related infectious factors were analyzed and the levels of 1,3-β-D-glucan detected were recorded in detail. RESULTS Among 128 cases of patients with pulmonary fungal infection, the fungi positive rate was 100.00% after fungal culture. A total of 128 strains of fungi were isolated, of which 56 strains were Candida albicans accounting for 67.19%, 19 cases were Candida smooth accounting for 14.84%. The main underlying diseases in 128 cases of pulmonary fungal infection were mainly chronic cardiovascular disease. Long-term use of antibiotics, long-term use of hormone and long-term use of immunosuppressive agents were related factors of pulmonary fungal infection in patients (P<0.001). The levels of alveolar irrigation and 1,3-β-D-glucan in plasma in fungal infection group were (589.23±312.42)ng/L and (356.45±231.24) ng/L, which were significantly higher than (121.36±57.39) ng/L and (74.23±43.28) ng/L in bacterial infection group and in non-infection group (P<0.001). The positive rate of CT diagnosis of pulmonary fungal infection was 69.53%, and was 87.50% by using alveolar lavage fluid. The positive rate of pulmonary fungal infection by CT combined with bronchoalveolar lavage fluid was 96.88%, which was better than a single method (P<0.001). CONCLUSION The causes and types of pulmonary fungal infection in patients are various. The use of CT combined with bronchoalveolar lavage fluid detection has important clinical reference value for the diagnosis of pulmonary fungal infection, especially early diagnosis and treatment.