Abstract: OBJECTIVE To explore pathogenic characteristics and related factors of nosocomial infections in patients with severe hepatitis. METHODS A total of 224 patients with severe hepatitis were selected from Jan. 2014 to Dec. 2016. The clinical data of the patients were analyzed retrospectively. The risk factors of infection were analyzed, and the identification of pathogenic bacteria was carried out. RESULTS Of the 224 patients with severe hepatitis, 47 patients were infected, and 53 cases were infected, the infection rate was 20.98%, and the case infection rate was 23.66%. The infection site was mainly in the abdominal cavity. Totally 134 strains of pathogens were cultivated. Among them, 68 strains were gram-negative bacteria accounting for 50.75%, 49 strains were gram-positive bacteria accounting for 36.57%, and 17 strains were fungi accounting for 12.69%. The results showed that the length of hospitalization, invasive operation, the use of antimicrobial agents and diabetes mellitus were related factors of nosocomial infections in patients with severe hepatitis. CONCLUSION There are many related factors in the nosocomial infections of patients with severe hepatitis, and the plan should be formulated in accordance with the individualized principle, taking targeted preventive measures.