Abstract: OBJECTIVE To probe into the monitoring results of pathogens and drug resistance of infection patients in emergency observation ward. METHODS From Jan. 2014 to Dec. 2015, 1395 patients in emergency observation ward in the hospital were selected as clinical subjects. The pathogens and the monitoring results of drug resistance of patients in emergency observation ward were analyzed. RESULTS Among 1395 patients in emergency observation ward, there were 586 cases with secondary infection, and the infection rate was 42.01%. Among 586 cases with secondary infection, there were 617 strains of non-repeat pathogens, including 341 strains of gram-negative bacteria, accounting for 55.27%, 250 strains of gram-positive bacteria, accounting for 40.52%, and 26 strains of fungi, accounting for 4.21%.Drug sensitivity results showed that, the major gram-negative bacteria showed high resistance to cefoperazone/sulbactam and piperacillin/tazobactam, and the drug resistant rates were more than 80%; while were highly sensitive to cefotaxime. The main gram-positive bacteria showed high resistance to penicillin G and sulfamethoxazole/oxazole, and the drug resistant rates were more than 80.00%; while were highly sensitive to vancomycin and teicoplanin. CONCLUSION The distribution of pathogens in the emergency observation ward was mainly gram-negative bacteria. The clinical intervention should select antibiotics based on the results of drug resistance monitoring.