Abstract: OBJECTIVE To explore the effect of urokinase combined with heparin sealing tube on the prevention of catheter infection in hemodialysis patients. METHODS A total of 72 patients treated by hemodialysis center in our hospital from Apr. 2015 to Apr. 2016 were selected. The patients were randomly divided into observation group and control group, with 36 cases in each group. The control group was treated with heparin saline, and the observation group was treated with regular urokinase combined with heparin sealing. The patients were observed and observed for 1 year. The clinical data, incidence of catheter infection during observation period, pathogens, coagulation function related indexes before and after treatment and catheter patency of two groups of patients were compared. RESULTS The incidence of ductal infection at the catheterization stage in the observation group was 2.78% (1/36), which was significantly lower than 19.44%(7/36) in the control group (P=0.024). There was no significant difference in coagulation function indexes between the two groups after treatment. The catheter embolization rate in the observation group was 5.56% (2/36), which was significantly lower than 36.11% (13/36) in the control group (P=0.001). The levels of catheter dialysis blood flow and urea clearance index in the two groups after treatment were all increased, and the catheter venous pressures were decreased. The catheter dialysis blood flow, urea clearance index and catheter venous pressure of the observation group were (257.65±34.59)ml/min, (1.53±0.64) and (91.89±14.52)mmHg, which were significantly lower than those of the control group (P<0.05) . CONCLUSION The combination of regular urokinase and heparin sealing can significantly prevent bacterial infection, which can reduce the incidence of catheter infection and improve the effect of dialysis.