Abstract: OBJECTIVE To probe into and analyze the distribution of common multidrug-resistant bacteria, risk factor and countermeasure of diabetic foot infection. METHODS A total of 268 cases of diabetic foot patients from Jan. 2014 to Dec. 2015 were selected as clinical subjects from the hospital. The distribution and drug resistance of common multidrug-resistant bacteria in patients with diabetic foot infection and the risk factores of infection were analyzed. RESULTS Totally 121 cases were with multidrug-resistant bacterial infection among 268 cases of diabetic foot patients, and the infection rate was 45.15%. Totally 149 strains of multidrug-resistant bacteria were detected,of which 75 strains of gram-negative bacteria accounted for 50.34%, mainly Pseudomonas aeruginosa and Escherichia coli, and 74 strains of gram-positive bacteria accounted for 49.66%, mainly Staphylococcus aureus. The gram-negative bacteria showed higher resistance to cefoperazone, and the drug resistant rates were more than 60.00%, while they were sensitive to meropenem and imipenem. The gram-positive bacteria showed higher resistance to erythromycin, and the drug resistant rate was 94.20%, while they were sensitive to vancomycin and teicoplanin. The diabetes course, the number of hospitalization of the same wound every year, combined with osteomyelitis, neurotoxic wound, and the previous use of antimicrobial drugs were risk factors of diabetic foot patients with multidrug-resistant bacterial infection (P＜0.05). CONCLUSION Antibiotics should be selected scientifically according to the specific drug resistance, and active nursing measures should be taken for patients with infection related factors.