Abstract: OBJECTIVE To investigate the effect of combined detection of interleukin - 6 (IL-6) and interleukin - 8 (IL-8) in the diagnosis of intrauterine infection. METHODS A total of 60 patients of full-term premature rupture of membranes (premature rupture of membranes, PROM) in our hospital from Sept. 2011 to Jan. 2015 were selected, and were randomly divided into PROM infection group (21 cases) and PROM uninfected group (39 cases) according to the intrauterine infection situation, IL-6 and IL-8 levels were taken from venous blood or amniotic fluid for determination, and 60 cases of full-term pregnant women with outpatient regular antenatal examination were set as control group. The relationships between IL-6 and IL-8 levels in peripheral blood and PROM chorioamnionitis were analyzed. RESULTS Levels of IL-6 and IL-8 from peripheral venous blood and amniotic fluid in the PROM infection group were(532.16±93.29)pg/mL, (567.84±134.15)pg/mL and (781.25±124.05)pg/mL, (794.63±118.64)pg/mL, respectively, which were significantly higher than (297.81±86.39)pg/mL, (304.56±98.67)pg/mL and (562.37±105.82)pg/mL, (589.32±124.55)pg/mL in the PROM uninfected group and the control group (P<0.05). Levels of IL-6 and IL-8 in peripheral venous blood and amniotic fluid in severe infection group were(641.59±104.25)pg/mL, (689.35±114.02)pg/mL and (849.63±130.47)pg/mL, (889.72±110.91)pg/mL,which were significantly higher than those in moderate infection group and mild infection group (P<0.05). With the extension of treatment time in patients with PROM infection, the levels of IL-6 and IL-8 in peripheral venous blood were gradually decreased (P<0.05). The combined detection of IL-6 and IL-8 had advantages in sensitivity, specificity, positive predictive value and negative predictive value. CONCLUSION Levels of IL-6 and IL-8 in peripheral blood can be used as an important index for diagnosis and evaluation of he severity of PROM intrauterine infection, and the combined detection of IL-6 and IL-8 has high sensitivity and specificity for prediction of PROM intrauterine infection.