Abstract: OBJECTIVE To understand the clinical characteristics of ICU multidrug-resistant bacteria nosocomial infection and the effects of cluster intervention on its prevention and control. METHODS A total of 1460 patients treated in ICU from Dec. 2014 to Jan. 2016 were selected, and were randomly divided into intervention group and control group, with 730 cases in each group. The distribution characteristics and drug susceptibility of the pathogens were analyzed. Patients in control group were treated with the general routine nursing intervention of the ICU ward, and patients in intervention group were given the relevant cluster intervention measures on the basis of control group. The days of hospitalization and prognosis of the two groups of patients were observed. RESULTS Among the 1460 patients who were treated in the ICU, 221 cases were infected, and the infection rate was 15.14%. There were 57 cases in intervention group, which was less than 164 cases in control group (P＜0.05). There were 246 cases of infections, and the infection rate was 16.85%. In 246 cases of infections, 87 cases were infected in intervention group, which was less than 157 cases in control group (P<0.05). Among them, 67 cases were multi drug resistant and multidrug-resistant rate was 4.59%. The main infection site was respiratory tract. Totally 498 pathogens were detected in 246 cases of ICU samples, including 369 strains of gram-negative bacteria accounting for 74.10%, 108 strains of gram-positive bacteria accounting for 21.69% and 21 strains of fungi accounting for 4.22%. Gram-negative bacteria had some resistance to the commonly used antibiotics in clinical practice, of which the resistant rates of Acinetobacter baumannii to ampicillin, ceftriaxone, cefazolin and nitrofurantoin were 100%, the resistant rate of Klebsiella pneumoniae to ampicillin was 100%, the resistant rates of Pseudomonas aeruginosa to ampicillin and ampicillin/sulbactam and Escherichia coli to ampicillin, ceftriaxone and cefazolin were 100.00%, and the resistant rates of Staphylococcus aureus to penicillin G, erythromycin and ampicillin/sulbactam,and Staphylococcus epidermidis to penicillin G were as high as 100.00%. The main gram-positive bacteria were more sensitive to furazolidin and vancomycin. The length of stay in intervention group was (32.34±5.70) d, which was less than (57.82±9.80) d in control group (P<0.05). The intervention group was significantly better than the control group in the treatment outcome (P<0.05). CONCLUSION For ICU multidrug-resistant nosocomial infection, it is necessary to strengthen the monitoring of drug-resistant strains and research on the related drug resistance genes, and adopt reasonable and effective cluster intervention and sensitive antimicrobial therapy to reduce the production of resistant strains and avoid its popularity and spread in the hospital.