Abstract: OBJECTIVE To know the current situation of disinfection quality in a 3A hospital in Ningbo, so as to provide evidence for reinforcing disinfection monitoring management and improving the disinfection quality in the hospital. METHODS Field sampling and microbiological detection methods were used to monitor the indoor air in hospital, medical staff hands, object surface, medical instruments, endoscopes cleaning, pressure steam sterilizer, hemodialysis fluid and hospital sewage from Jan. 2013 to Dec. 2016, and the monitoring results were analyzed. RESULTS A total of 1295 samples were collected during the year 2013 to 2016, and 1199 were eligible with the total qualified rate of 92.59%. The qualified rates during the four years were 93.23%, 95.10%, 90.71%, and 91.12%, respectively. The qualified rate of environment type Ⅰ was 98.18%, which was obviously higher than that of type Ⅱ~Ⅳ of 69.23%. A total of 134 endoscopes were monitored, including 86 gastroscopes, 32 enteroscopes, 13 bronchoscopes and 3 cystoscopes. The results of the endoscopic cleaning and disinfection efficacy were all qualified. The qualified rate of hand hygiene was 85.56%, and that of surgical hand was 91.55%(65/71), which was significantly higher than 80.40%(160/199) of hygienic hand (P＜0.05). The ATP bioluminescence assay for evaluating the cleaning effect showed that the qualified rate of medical instrument was 35.62%, and annual variation was great. The chlorine dioxide monitoring results showed that the qualified rate of sewage was 78.43%, CONCLUSION The disinfection and sterilization effects of the hospital are good in general, but the qualified rates of hand hygiene, hospital sewage and endoscopes cleaning were relatively low, so more attention should be focus on these items, so as to improve the quality of disinfection and sterilization.