Abstract: OBJECTIVE To analyze the detection rate, SCCmec genotypes and drug resistance profiles of methicillin-resistant Staphylococcus aureus (MRSA) in our hospital, in order to provide reference for clinical prevention and treatment. METHODS Staphylococcus aureus (SAU) strains cultured and isolated in the microbiology laboratory of our hospital from 2014 to 2016 were collected, and MRSA isolates were identified according to their sensitivity to methicillin, and then SCCmec genotyping and drug susceptibility test were performed. RESULTS From 2014 to 2016, a total of 148 MRSA isolates was detected, and the average detection rate was 37.28%. SCCmec genotypes Ⅰ, Ⅱ Ⅲ and Ⅳ were found, with SCCmec Ⅲ type as the top common type, accounting for 72.30%. All MRSA isolates were resistant to penicillin and oxacillin, and sensitive to vancomycin, linezolid,tigecycline,quinupristin/dalfopristin, and nitrofurantoin. All of the isolates of SCCmec genotype Ⅰ were sensitive to the rest of the antibiotics. SCCmec genotype Ⅲ showed the widest antimicrobial resistance profiles. CONCLUSION The MRSA detection rate in our hospital is slightly lower than the national average level. The main epidemic genotype of the isolates is SCCmec Ⅲ. The detection rate of MRSA should be continuously monitored, so as to master the distribution of genotypes and the changes of antimicrobial resistance profiles.