Abstract: OBJECTIVE To investigate the application of evidence-based medicine in prevention of carbapenem-resistant Enterobacteriaceae (CRE) infection in patients with multiple myeloma, thus reducing the incidence of CRE in patients with multiple myeloma and improving the survival quality of patients. METHODS A total of 196 patients with multiple myeloma treated in our hospital from Jan.2014 to Jan.2017 were randomly divided into two groups (n= 98).The control group was given routine care, whereas the experimental group was given individualized evidence-based ursing on the basis of routine care.The main causes of infection in patients with multiple myeloma were analyzed by fishbone diagram.The incidence of CRE infection and the main source of CRE infection in patients with multiple myeloma were compared between the experimental group and the control group. RESULTS The incidence of CRE infection in the experimental group (1.020%) was significantly lower than that in the control group (7.142%)(P＜0.05).The specimens with CRE infection in the patients of the control group were mainly from sputum, accounting for 57.14% (4/7), and the dominant pathogen was Klebsiella pneumonia, accounting for 57.14% (4/7).The source of specimens with CRE infection in the patients of the experimental group was also sputum, and the pathogen was also K pneumonia. CONCLUSION Evidence-based research can effectively prevent the occurrence of CRE infection in patients with multiple myeloma, improve the quality of care, and thus improve the quality of life of patients.The effect is significant, and it is worth promoting in the clinic.