Abstract: OBJECTIVE To explore and analyze the influence of Ureaplasma urealyticum infection in pregnant women on pregnancy outcomes and related preventive measures. METHODS A total of 327 parturient women giving birth in the hospital from Jul. 2015 to Jun. 2017 were selected as the subjects in the clinical study. According to the U urealyticum test results of the prenatal cervical secretions, 98 cases with positive results were set as the observation group and 229 cases with negative results were set as the control group. The influence of U urealyticum infection in pregnant women on pregnancy outcomes and related preventive measures were comprehensively and deeply explored and analyzed. RESULTS The premature delivery rate was 19.39% (19/98) and rate of premature rupture of membranes was 35.71% (35/98) in the patients with ureaplasma urealyticum infection in the observation group, significantly higher than those of the control group (6.99% and 13.97%)(P＜0.05). In the patients with U urealyticum infection in the observation group, the incidence of low birth weight infants was 10.20% (10/98), neonatal jaundice was 14.29% (14/98), neonatal pneumonia was 9.18% (9/98), which were significantly higher than those of the control group (4.37%, 6.11% and 2.62%) (P＜0.05). CONCLUSION U urealyticum infection in pregnant women could increase the adverse pregnancy outcomes, and has certain impact on the perinatal health. In clinical, positive preventive interventions should be given to ensure the safety of pregnant women and perinatal infants.