Abstract: OBJECTIVE To analyze the effect of alanyl glutamine on glutathione (GSH) and glutamine (GIn) levels in sepsis patients and its clinical therapeutic effect. METHODS 94 patients with sepsis admitted in our hospital from Jan. 2015 to Jun. 2017 were selected, and divided into the control group and the experimental group according to the method of random number table. The control group was treated with conventional nutritional support, whereas the experimental group was treated with conventional nutritional support combined with continuous intravenous infusion of alanyl glutamine. The clinical therapeutic effect was analyzed for the two groups of patients. RESULTS After treatment, the levels of ALB, TF, GSH and GIn in the experimental group were significantly higher than those in the control group, while the levels of hs-CRP and LPS were significantly lower than those in the control group (P＜0.05). The durations of ventilator and antibiotic use in the experimental group were (4.75±1.66) and (5.29±2.24) days, respectively, and those in the control group were (7.98± 2.14) and (9.65±3.00) days, respectively. The incidence of multiple organ dysfunction syndrome was 12.77% in the experimental group, significantly lower than that in the control group (31.91%) (P＜0.05). CONCLUSION Alanyl glutamine treatment of sepsis can effectively improve the nutritional status of the patients, inhibit the expression of inflammatory factors, improve GSH and GIn levels, reduce organ damage, and improve the prognosis of patients, with high safety.