Abstract: OBJECTIVE To explore the risk factors of multidrug-resistant bacteria infection in the surgical sites of extremities fracture patients and provide guidance for clinical treatment. METHODS The clinical data of 922 patients with limbs fracture surgery from Jul. 2014 to Aug. 2017 in our hospital were collected. According to whether multidrug-resistant bacteria were detected, the patients were divided into multidrug-resistant bacteria group and non multidrug-resistant bacteria group. The patient's incisional secretion was collected, and the variety and number of multidrug-resistant strains of the patient were determined. Univariate analysis and multivariate analysis were carried out on the clinical data of the patients such as operation types, presence of implants, nutritional status, and operation time. RESULTS A total of 376 strains of pathogenic bacteria were isolated from the 86 patients with wound infection after limb fracture operation, among which 74 strains were multidrug-resistant bacteria, and the infection rate of multidrug-resistant bacteria was 19.68%. Multivariate logistic regression analysis results showed that the presence of implants, malnutrition, postoperative antibiotics use of ≥ 7 days, and plasma hemoglobin level of < 90 g/L were independent risk factors for multidrug-resistant bacteria infection in patients with limb fractures (P<0.05). CONCLUSION Multidrug-resistant bacteria infection is closely related to implants, surgical types, surgical incision length and other factors. Special attention should be paid to these factors in clinic.