Abstract: OBJECTIVE To investigate the clinical efficacy of sequential anticoagulant thrombolysis combined with mechanical ventilation in the treatment of acute lung injury associated with abdominal infection and acute pulmonary embolism. METHODS Forty patients with acute lung injury due to abdominal infection and acute pulmonary embolism admitted in our hospital from Jun.2015 to Aug.2017 were selected and randomly divided into two groups: experimental group (20 cases) and control group (20 cases).Patients in the control group were treated with conventional thrombolysis combined with antimicrobial drugs.The experimental group was treated with thrombolytic and sequential anticoagulation combined with mechanical ventilation on the basis of conventional antimicrobial therapy.The treatment of the two groups was compared. RESULTS The clinical efficacy of the experimental group (90.00%) was significantly better than that of the control group (70.00%) (χ²= 4.800, P＜ 0.05).After treatment, the blood gas indexes in both groups were improved as compared to that before treatment, and the improvement of the experimental group was significantly better than that of the control group (P＜ 0.05).A total of 44 strains of pathogens were isolated from the 40 cases of patients, among which there were 33 strains of gram-negative bacteria accounting for 75.00%, 10 strains of gram-positive bacteria accounting for 22.73%, and 1 strain of fungus accounting for 2.27%. CONCLUSION Sequential anticoagulant therapy with thrombolytic therapy combined with mechanical ventilation is effective in treating acute lung injury due to abdominal infection complicated with acute pulmonary embolism.It can quickly relieve various clinical symptoms of patients with high safety and has promotion value.