Abstract: OBJECTIVE To investigate the infection status of hepatitis B virus (HBV) among voluntary blood donors in this area. METHODS A total of 27556 blood samples collected from the blood collection sites during Jan. 2014 - Sep. 2017 were selected as the research objects. ELISA was used to detect hepatitis B virus surface antigen (HBsAg) in the blood samples collected, and NAT detection was conducted for the specimens that had no response to one or both of the above HBsAg diagnosis. RESULTS A total of 88 HBsAg-positive blood samples were detected in 27556 voluntary blood donors, the positive rate was 0.32%, and 22 cases were NAT positive, with a positive rate of 0.08%. HBsAg-positive and NAT-positive infection rates showed a decreasing trend year by year in 2014~2017. The positive rates of HBV in blood donors aged 18~29 years old, 30~49 years old and over 50 years old were 0.40%, 0.39% and 0.40%, respectively. The positive rates of HBV in male and female donors were 0.39% and 0.41%, respectively. There was no significant difference in HBV positive rate between voluntary blood donors of different age and gender. The positive rates of HBV in rural and urban blood donors were 0.22% and 0.48%, respectively. The positive rate of HBV in urban blood donors was significantly higher than that in rural areas (P<0.05). The positive rates of HBV in the first, second and third or more time blood donors were 0.90%, 0.26% and 0.23%, respectively. The positive rate of HBV in the first-time voluntary blood donors was significantly higher than those of the second and third or more time donors (P<0.05). CONCLUSION The positive rate of HBV in the voluntary blood donors in this area shows a decreasing trend, and there is a certain regional difference. Therefore, we should continue to increase the intensity of pre-screening and blood testing, consolidate the fixed blood donation population, ensure the safety of blood transfusion, and further reduce the risk of transfusion-transmitted HBV.