Abstract: OBJECTIVE To explore the clinical effect of the classified management system in central sterile supply department. METHODS The incidence of nosocomial infection that occurred between Jan 2015 to Dec 2016 was retrospectively analyzed, the classified management system started to be carried out in the central sterile supply department in 2016. The nursing quality, effect of disinfection of instruments and incidence of nosocomial infection were observed and compared before and after the classified management system was carried out. RESULTS The scores of safety identification ability, risk prevention awareness and service consciousness of the nursing staff were respectively (9.22±1.24)points, (9.41±1.62)points and (9.62±1.03)points after the classified management system was carried out, significantly higher than those before it was carried out (P<0.05). The qualified rate of cleaning, recleaning rate, qualified rate of disinfection and qualified rate of packaging of spot check instruments were respectively 96.88%(775/800),96.13%(769/800),97.38%(779/800)and 97.75%(782/800)after the classified management system was carried out, significantly higher than those before it was carried out (P<0.05). The departments' satisfaction with the central sterile supply department was 94.44%(34/36)after the classified management system was carried out, significantly higher than 86.11%(31/36) before it was carried out (P=0.017). The incidence rate of surgery-related nosocomial infection was 16.71‰(755/45 191) after the classified management system was carried out, significantly lower than 19.34‰(735/37 998) before it was carried out (P=0.004). CONCLUSION The classified management system can not only improve the comprehensive quality and nursing quality of the nursing staff but also reduce the risk of nosocomial infection, and it has certain clinical value.