Abstract: OBJECTIVE To develop a kind of combined isolation screen and observe its air-tightness, and to explore its feasibility for the terminal disinfection of open hospital rooms. METHODS The combined isolation screen was composed of brackets and the detachable isolation module. The angle ferroalloy was used as bracket of isolation screen, which was fixed with the screw on the wall and roof and liked "U", and the light waterproof cloth was used as the main material of the removable isolation module, which was sewed by double layers and soft magnet on the edge. The isolation module was tightly fixed to the bracket by the magnetic force of soft magnet and ferroalloy. The lower edge was compacted with sandbags. The finished combined isolation screen was completely separated 47m³ simulated room into two different areas. And 35% food-grade hydrogen peroxide solution was passed into disinfection area after vaporization according to the standard of 6 g/m³ for disinfection of 1h. The gas concentrations of two areas were monitored continuously. RESULTS The combined isolation screen was composed of ferroalloy bracket and isolation module with soft magnet on the edge by magnetic force, which was simple in production, convenient in installation and low in cost. It had little effect on the layout and function of hospital rooms and could be used for alternate disinfection in different areas of rooms. In simulated disinfection experiments, the concentration of hydrogen peroxide in the disinfection area increased continuously with the increase of disinfection tine during the sterilization stage, and it reached the maximum value of (139.03±3.51)ppm after 1h. It decreased rapidly during the decomposition stage.The gas leakage in the non-disinfection area gradually accumulated, and then the decomposition decreased, which was always lower than the safety limit of 1ppm. CONCLUSION The combination isolation screen is simple in production, convenient in installation with good air tightness, and it can separate hospital rooms in different areas for alternate disinfection completely and physically, without affecting normal clinical treatment work, which has a certain clinical application value.