Abstract: OBJECTIVE To investigate the current status of invasive yeast infection in 15 tertiary teaching hospitals in Hebei province from 2016 to 2017 so as to understand the epidemiological characteristics of the invasive yeast infection in Hebei province. METHODS The invasive yeast strains that were isolated from clinical department were collected and statistically analyzed, and the strains were preliminarily screened and identified by using Chromogenic medium CHROMagar and Compact YST card, all of the strains were re-examined and identified by using microorganism mass spectrum, and the ITS sequencing analysis was performed for the difficult and rare strains. RESULTS Of totally 268 strains of invasive yeast-like fungi that were isolated from 15 tertiary hospitals between 2016 and 2017, 112 (41.79%) were Candida albicans, 52 (19.40%) were Candida tropicalis, and 38 (14.18%) were Candida parapsilosis.131 (48.88%) were isolated from blood specimens, 137 (51.12%) strains were isolated from aseptic body fluid specimens.69 (25.75%) strains were isolated from internal medicine departments (hematology department, oncology department, respiratory medicine department), 67 (25.00%) strains were isolated from surgical departments (general surgery department, neurosurgery department), and 55 (20.52%) strains were isolated from ICU.The correct rate of reexamination of the strains was 83.96% (225/268). CONCLUSION C.albicans remain the predominant species of invasive yeast, however, the incidence of infection caused by non-C.albican strains is increased unevenly.Mass spectrometry may effectively raise the correct rate of identification of difficult strains so as to provide etiological basis for clinical treatment.