Abstract: OBJECTIVE To investigate the prevalence of device-related infection in comprehensive ICU of a district of Shanghai so as to put forward the targeted prevention and control measures. METHODS By means of retrospective survey, the data of targeted surveillance of nosocomial infections were collected from comprehensive ICUs of 5 hospitals from 2015 to 2017, the statistical analysis was performed by using SPSS21.0 software, and the incidence of nosocomial infections and device-related infection in the ICU patients was analyzed. RESULTS Totally 4788 patients were enrolled in the study, with the total hospitalization duration 50418 days; 434 (510 case-times) patients had nosocomial infections, the incidence rate of nosocomial infections was 9.06%, the daily infection rate was 10.12‰, and the adjusted daily infection rate was 4.50‰.The patients with respiratory system infection were dominant among the patients with nosocomial infection, accounting for 71.76%; totally 221 case-times of patients had the device-related infection, accounting for 43.33% among the patients with nosocomial infections, and the percentage showed downward trend in the three consecutive years (χ²=24.44,P＜0.001).Among the patients with device-related infection, the incidence rate of ventilator-related pneumonia (VAP) was 6.01‰, the incidence rate of urinary catheter-related urinary tract infection was 1.93‰, the incidence rate of central catheter-related bloodstream infection was 0.68‰, and the incidence rate of VAP was the highest.A total of 335 strains of pathogens were isolated from the patients with the device-related infection, 67.16% of which were gram-negative bacteria, and 19.10% were gram-positive bacteria; Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae and Acinetobacter baumannii were the predominant species of pathogens. CONCLUSION The patients with device-related infection are dominant among the patients with nosocomial infections in the ICU.It is necessary to continuously conduct the targeted surveillance of nosocomial infections in ICU and continue to strengthen the information construction of nosocomial infections.