Abstract: OBJECTIVE To investigate the distribution and drug resistance of pathogens causing nosocomial infections in a tertiary general hospital of Inner Mongolia from 2015 to 2017 so as to provide theoretical basis for reasonable clinical use of antibiotics. METHODS The patients with nosocomial infections who were treated in the hospital from Jan 1, 2015 to Dec 31, 2017 were recruited as the study objects, and the distribution and drug resistance of pathogens causing the nosocomial infection were retrospectively analyzed. RESULTS A total of 3063 strains of pathogens were isolated from 2283 patients with the infections, 72.61% of which were gram-negative bacteria, and 27.39% were gram-positive bacteria; Escherichia coli, Acinetobacter baumaannii, Pseudomonas aeruginosa, Enterococcus faecium and Klebsiella pneumoniae ranked the top five species of pathogens.Lower respiratory tract, blood and urinary tract ranked the top 3 infection sites.The E.coli strains were most resistant to ampicillin but were susceptible to imipenem; The K.pneumoniae strains were highly resistant to piperacillin and ampicillin; the A.baumannii strains were highly resistant to most of the antibiotics, and the drug resistance rate of the strains to levofloxacin was low; the drug resistance rates of the P.aeruginosa strains to ampicillin and ampicillin-sulbactam approximated 100.00%; the drug resistance rates of the E.faecium and Enterococcus faecalis to clindamycin were 100.00% in the three years, while the strains were susceptible to tigecycline; the Staphylococcus epidermidis and Staphylococcus aureus strains were highly resistant to penicillin and cefoxitin, while the strains were highly susceptible to linezolid and tigecycline. CONCLUSION The species of pathogens causing the nosocomial infection are complex, the pathogens are highly drug-resistant, and it is necessary to strengthen multidisciplinary collaboration and take effective measures so as to prevent the spread of the drug-resistant strains in hospitals.