Abstract: OBJECTIVE To explore the causes of outbreak of Enterobacter cloacae nosocomial infection in neonates, observe the effect and advantages of multidisciplinary team (MDT) intervention and put forward the prevention and control countermeasures. METHODS The clinical data of 12 neonates with clustered fever were analyzed, the result of the epidemiological survey was observed, and the result of specimens culture was investigated. RESULTS Of the 12 neonates with clustered fever, 9 were positive for blood culture, showing the growth of E.cloacae. The nosocomial infection outbroke around the spring festival, all of the neonates had underlying diseases, including 8 cases of premature infants and 5 cases of low body weight. CONCLUSION The nosocomial infection induced by the E.cloacae is associated with the low immunity of neonates and premature infants, use of antibiotics, environment, isolation and disinfection, hand hygiene, monitoring of nosocomial infection in wards and biological characteristics of E.cloacae. The emergency plan was launched in time, and the MDT collaborative joint intervention really played the combined role of 1+1>2. Reconstructing the unreasonable layout and process of neonatology; constructing ground, wall and facilities that are easy to clean and disinfect effectively; rationally deploying human resources, strengthening all-round supervision of neonatal sense of control, processes, and intensifying training and standard prevention to ensure that all the prevention measures such as hand hygiene, cleaning and disinfection of air and object surfaces were carried out in place are the key links for the prevention and control of the E.cloacae nosocomial infection in the neonates. The MDT intervention can achieve remarkable effect on the prevention of the E.cloacae nosocomial infection in the neonates.