临床分离鲍氏不动杆菌生物膜形成能力与耐药性关系研究

Relationship between ability of biofilm formation and drug resistance of clinical Acinetobacter baumannii isolates

    摘要
  • 摘要: 目的 研究鲍氏不动杆菌生物膜形成能力与耐药性的关系。方法 收集2016年7月-2018年2月临床分离的85株鲍氏不动杆菌菌株,检测其药敏及生物膜形成能力,采用PCR技术检测鲍氏不动杆菌生物膜相关基因bap、abaI、bfs、bfmS、bfmR、csuA、csuE、csuAB。结果 85株鲍氏不动杆菌对替加环素、米诺环素、头孢哌酮/舒巴坦的耐药率分别为11.76%、23.53%、25.88%,耐药率最高的为环丙沙星和头孢吡肟,均为71.76%,其中耐药组(包括多重耐药和泛耐药菌)58株占68.24%,敏感组27株占31.76%; 所有实验菌株均能形成生物膜,敏感组鲍氏不动杆菌生物膜形成能力强于耐药组细菌(Z=-6.391,P＜0.001); 85株鲍氏不动杆菌生物膜相关基因bfmS、bfmR、bfs、csuE、csuA、csuAB、abaI、bap的检出率分别为98.82%、98.82%、95.29%、90.59%、89.41%、89.41%、87.06%、78.82%,耐药组基因bap、abaI、bfs的检出率高于敏感组(P＜0.05),其他基因在两组间检出率无差异。结论 鲍氏不动杆菌耐药现象严重,敏感组细菌生物膜形成能力强于耐药组,多种基因参与细菌生物膜的形成。

     

    Abstract: OBJECTIVE To study the relationship between the ability of biofilm formation and the drug resistance of Acinetobacter baumannii. METHODS A total of 85 clinical A.baumannii isolates were collected from Jul 2016 to Feb 2018, the drug susceptibility rates and the ability of biofilm formation of the strains were tested, and the biofilm-related genes of the A.baumannii strains such as bap, abaI, bfs, bfmS, bfmR, csuA, csuE and csuAB were detected by using PCR. RESULTS The drug resistance rates of the 85 strains of A.baumannii to tigecycline, minocycline and cefoperazone-sulbactam were 11.76%, 23.53% and 25.88%, respectively; the drug resistance rates to ciprofloxacin and cefepime were the highest (71.76%).There were 58 (68.24%) strains of drug-resistant A.baumannii (including multidrug-resistant strains and pandrug-resistant strains) and 27 (31.76%) strains of drug-sensitive A.baumannii.All of the test strains had the ability of biofilm formation, and the ability of biofilm formation of the drug-sensitive strains was greater than that of the drug-resistant strains (Z=-6.391,P＜0.001).The detection rates of bfmS, bfmR, bfs, csuE, csuA, csuAB, abaI and bap were 98.82%, 98.82%, 95.29%, 90.59%, 89.41%, 89.41%, 87.06% and 78.82%, respectively.The detection rates of bap, abaI and bfs of the drug-resistant strains were significantly higher than those of the drug-sensitive strains (P＜0.05); there were no significant differences in the detection rates of other genes between the two groups. CONCLUSION The A.baumannii strains are highly drug-resistant, the ability of biofilm formation of the drug-sensitive strains is greater than that of the drug-resistant strains, and multiple genes get involved in the formation of bacterial biofilm.

     

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