Abstract: OBJECTIVE To investigate the clinical distribution and drug resistance of carbapenem-resistant Enterobacteriaceae (CRE) strains and explore the strategies for clinical prevention and control of CRE infection. METHODS A total of 85 patients who were diagnosed with CRE infection and hospitalized from Jan 2011 to Jun 2018 were recruited as the study objects, and the incidence of CRE infection was observed. RESULTS The neurosurgery department was the predominant department where the CRE strains were isolated, accounting for 30.59%, followed by the hematology department and critical medicine department.36.47% of the CRE strains were isolated from sputum specimens, 25.88% from urine specimens, 23.53% from blood specimens.Klebsiella pneumoniae, Escherichia coli and Enterobacter cloacae were dominant among the isolated CRE strains, accounting for 51.76%, 32.93% and 8.24%, respectively.All of the 85 CRE isolates were resistant to imipenem, ceftriaxone, cefotaxime and amoxicillin-clavulanic acid, while the drug resistance rates to cefmetazole and ceftazidime.The constituent ratio of the patients with CRE infection was increased with the age, the patients aged more than 60 years old occupied the greatest proportion, accounting for 36.47%, and there was significant difference among the age groups (χ²=22.010,P＜0.001).The male patients with CRE infection were more than then female patients (χ²=47.650,P＜0.001).The isolation rate of CRE strains was highest in the summer (June-August) (χ²=10.340,P=0.010). CONCLUSION The neurosurgery department, hematology department and critical medicine department are the department at high risk of CRE infection.The respiratory system, urinary system and blood system are major infection sties of the patients with CRE infection.K.pneumoniae and E.coli are the predominant species of CRE.The CRE strains are highly multidrug-resistant, the proportion of the elderly patients with CRE infection is relatively high, and the CRE infection is highly prevalent in summer.