Abstract: OBJECTIVE To understand the distribution and drug resistance of clinical strains isolated from the hospital in recent two years so as to provide theoretical basis for reasonable clinical use of antibiotics. METHODS The bacteria that were isolated from the clinical specimens of the hospital from Jan 2016 to Dec 2017 were identified by using conventional method and API. The drug susceptibility testing was performed with the use of disk diffusion method, and the result of the drug susceptibility testing was interpreted by CLSI M 100 S27 of 2017 edition. RESULTS Of totally 2909 strains of bacteria isolated, 49.57% were isolated from wound secretions, 32.31% were isolated from respiratory tract specimens, 10.48% were isolated from urine specimens, and 1.72% were isolated from blood specimens. There were 2168 (74.53%) strains of gram-negative bacteria and 741 (25.47%) strains of gram-positive bacteria. Klebsiella pneumoniae accounted for 13.78%, Escherichia coli 12.48%, Staphylococcus aureus 9.63%, coagulase-negative Staphylococcus 9.52%, Pseudomonas aeruginosa 8.87%, Enterobacter cloacae 8.70%, Acinetobacter baumannii 6.46%. The result of the drug susceptibility testing indicated that the drug resistance rates of the Enterobacteriaceae strains to cefoperazone-sulbacterm and piperacillin-tazobactam were respectively 2.05% and 4.81%, the drug resistance rates to carbapenems ranged between 0.8% and 2.2%; the drug resistance rates of the P.aeruginosa strains to imipenem and meropenem were 9.71% and 12.51%, respectively, and the drug resistance rates of the A.baumannii strains were 21.44% and 20.32%, respectively; the drug resistance rates of the S.aureus and coagulase-negative Staphylococcus to penicillin were 92.63% and 92.16%, respectively. CONCLUSION There is certain difference between the surveillance data of bacterial resistance in special hospitals and the statistical data of national antimicrobial resistance surveillance network. Empirical anti-infection treatment should not completely rely on the data of the national surveillance network. It is necessary for the special hospital to reasonably use antibiotics based on the distribution and drug resistance of the strains isolated from the hospital.