Abstract: OBJECTIVE To investigate the expression of stress-related microRNA（microRNA） in patients with diabetic foot complicated with infection.METHODS 78 patients with diabetic foot complicated with infection who were treated in hospital from Mar. 2017 to Jul. 2018 were selected as the experimental group. At the same time, 78 patients with diabetes mellitus complicated with diabetic foot infection in the same period were selected as the control group. The clinical data were collected and the levels of total cholesterol（TC）, triglyceride（TG）, high density lipoprotein cholesterol（HDL-C）, low density lipoprotein chotesterol（LDL-C）, fasting blood glucose（FPG） and glycated hemoglobin A1c（HbA1c） were measured. The stress-related microRNA was screened by bioinformatics method, and the expression level of stress-related microRNA was detected by real-time fluorescence quantitative PCR.RESULTS TC, TG, LDL-C, FPG and HbA1 c in the experimental group were（5.67±1.21） mmol/L,（3.01±0.72） mmol/L,（3.09±0.76） mmol/L,（9.39±2.70）mmol/L and（7.21±1.32）%, higher than those in the control group（P＜0.05）. Corticotropin-releasing hormone, interleukin-6（IL-6）, plasma adrenaline, norepinephrine and cortisol in the experimental group were（5.67±0.51） μg,（134.27±14.23） ng/L,（157.82±43.29） ng/L,（423.18±93.27） ng/L and（223.29±36.37） μg/L, respectively, higher than those of the control group（P＜0.05）. The corticotropin-releasing hormone was negatively correlated with microRNA-142 level, while IL-6, adrenaline and norepinephrine were positively correlated with Let-7 b, and negatively correlated with microRNA-142 level（P＜0.05）. The levels of cortisol were positively correlated with microRNA-144, miR29 a and Let-7 b, and negatively correlated with microRNA-142（P＜0.05）. In the experimental group, corticotropin-releasing hormone, plasma adrenaline and norepinephrine were positively correlated with the level of Let-7 b. Cortisol was also positively correlated with microRNA-144 and microRNA-29 a levels, but IL-6 level was negatively correlated with microRNA-142（P＜0.05）.CONCLUSION Compared with the control group, the diabetic foot patients of the experimental group showed increased levels of microRNA-144 and Let-7 b, while the microRNA-142 level decreased. microRNA-144, Let-7 b and microRNA-142 may be regulatory factors for occurrence and development of diabetic foot under chronic stress. It provides a certain direction for the diagnosis and prevention of stress-related microRNA in patients with diabetic foot, which is worth further exploration.