Abstract: OBJECTIVE To analyze the influencing factors for multidrug-resistant organisms (MDROs) infections in intensive care unit (ICU) patients. METHODS A total of 303 ICU patients with drug-resistant bacteria infection who were treated in The Fifth Hospital Affiliated to Zhongshan University from Jan 2016 to Dec 2017 were enrolled in the study. The distribution of pathogens causing the infection was observed, the drug resistance rates of major species of pathogens were analyzed, the enrolled patients were divided into the MDROs infection group with 187 cases and the non-MDROs infection group with 116 cases according to the result of drug susceptibility testing, and the influencing factors for the MDROs infection were analyzed. RESULTS Totally 446 strains of pathogens were isolated from the 303 patients with infection, 80 (17.94%) of which were Acinetobacter baumannii. The drug resistance rate of Pseudomonas aeruginosa strains to sulfamethoxazole-trimethoprim was the highest; the drug resistance rates of A. baumannii strains to cefotaxime and piperacillin were the highest; the drug resistance rate of Staphylococcus aureus strains to gentamicin was the highest; the drug resistance rate of Escherichia coli strains to sulfamethoxazole-trimethoprim was the highest. The influencing factors for the MDROs infection included gastric tube indwelling time, urinary catheter indwelling time, length of hospital stay, types of antibiotic, deep venous catheter and more than 48 hours of mechanical ventilation. Logistic regression analysis showed that the gastric tube indwelling time, urinary catheter indwelling time, length of hospital stay, deep venous catheter indwelling time, types of antibiotics and more than 48 hours of mechanical ventilation were the independent influencing factors for the MDROs infection. CONCLUSION The incidence rate of MDROs infection is high among the ICU patients, and it is necessary to conduct effective guidance and supervision for use of antibiotics, treatment and nursing so as to reduce the incidence of infection.